LE3 .A278 2012

2012

Mockford, Steve
Lefebvre, Jose

Acadia University

Bachelor of Science

Honours

Biology

Biology

Worldwide, turtle populations are diminishing at astonishing rates. To date, a large amount of research has focused on the female role in reproduction in hopes of improving our knowledge of the reproductive ecology of these reptiles. Although this information is useful, further investigation on the male reproductive dynamic is essential for planning conservation strategies. The four freshwater turtle species found within Nova Scotia are not exempt from the declining trends. With three of the four species being protected at the federal or provincial level, research into the males’ contribution to the gene pool is crucial. In order to properly study male fertility, sperm quality and its implications on population growth and stability, sperm samples need to be obtained. To date, there are three recognized methods for sperm collection from turtles-each of them being either highly invasive or lethal. This study developed and tested a new semen extraction technique, using vibration on the plastron and carapace to induce erection and ejaculation. This technique is minimally invasive and allows semen to be collected in situ. The technique was tested on 12 Blanding’s turtles (Emydoidea blandingii), 17 wood turtles (Glyptemys insculpta) and 30 eastern painted turtles (Chrysemys picta picta) captured by hand or using baited three-hoop traps between June and August 2011. The technique was successful in inducing (pre)ejaculate fluid for collection in 100% of E. blandingii, 11.8% of G. insculpta and 13.3% of C. picta picta. It was determined that the number of successful trials in E. blandingii was statistically different in comparison to the other two species. Extraction attempts on C. picta picta were significantly shorter in duration than attempts made on E. blandingii and G. insculpta. This non-lethal, non-invasive technique has shown encouraging results, particularly for E. blandingii.

The author retains copyright in this thesis. Any substantial copying or any other actions that exceed fair dealing or other exceptions in the Copyright Act require the permission of the author.

https://scholar.acadiau.ca/islandora/object/theses:923