LE3 .A278 2005

2005

Roscoe, Sharon

Acadia University

Bachelor of Science

Honours

Chemistry

Chemistry

Protein-containing calcium phosphate coatings (protein/brushite) were prepared by electrochemically assisted co-precipitation onto hydroxyapatite (HA) coated Ti-6Al-4 V surfaces. The three model proteins that were used were lysozyme, holo-􀄮- lactalbumin and lactoferrin. Electrochemically assisted co-precipitation of holo-􀄮-lactalbumin/brushite and lactoferrin/brushite coatings onto HA resulted in increased protein inclusion compared to simple adsorption. The electrochemically assisted co-precipitation of lysozyme/brushite coatings onto HA showed a decrease in the amount of protein incorporated as compared to simple adsorption. For all three protein/brushite coatings, the cumulative percentage of protein released from the electrochemically assisted co-deposited coatings was less than that of the simply adsorbed coatings. The release of protein from the electrochemically assisted co-precipitated holo-􀄮-lactalbumin/brushite coatings onto HA was much more rapid than the release of protein from the simply adsorbed coatings. Holo-􀄮-lactalbumin was the only protein that showed a burst release from the electrochemically deposited coatings. Both the lysozyme/brushite and lactoferrin/brushite coatings gave release profiles which were markedly slower than their simply adsorbed counterparts. Thus, the electrochemically assisted co-precipitation technique was able to provide an efficient method of protein incorporation at physiological temperature. The subsequent slow release of protein from the electrochemically deposited coatings shows potential for the sustained release of growth factors and other agents required for metallic implant fixation.

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