Differential expression of antifreeze gylcoprotein genes among tissue types in Atlantic tomcod Microgadus tomcod
LE3 .A278 2022
Bachelor of Science
Atlantic tomcod Microgadus tomcod synthesize antifreeze glycoproteins (AFGPs) that protect body tissues from freezing by inhibiting ice crystal growth. Other antifreeze producing fishes exhibit tissue specific differential antifreeze protein production. In this study, the expression of MAK14 and RAB14 genes coding for antifreeze glycoproteins 1-4 was examined among four tissue types, liver, skin, gill, and head kidney in Atlantic tomcod. Nucleic acids were extracted from samples using silica membrane and phenol-chloroform techniques. The Polymerase Chain Reaction was used to amplify target sequence and the resulting amplicons were sequenced. Sequence data was entered into a BLASTn search engine and identified MAK14 and RAB14 gene in Microgadus tomcod as the top match. We used a reverse transcription polymerase chain reaction and real time quantitative polymerase chain reaction to explore expression of MAK14 and RAB14 genes among tissue types.
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