Exploring expression of tp53 in zebrafish, Danio rerio, in response to exogenous cannabinoids and stress
LE3 .A278 2021
Bachelor of Science
The endocannabinoid system (ECS) is an essential signalling network in vertebrates that includes essential receptors, enzymes, and transporter proteins of the central and peripheral nervous systems. The ECS includes cannabinoid receptors divided into CB1R and CB2R subtypes, which are part of the G-protein-coupled receptors (GPCRs) family. These, in turn, activate the c-JUN N-terminal kinases (JNK) pathway. The JNK pathway facilitates a range of abiotic and biotic stress effects that regulate physiological processes such as inflammation, neuronal functions, cell death and survival pathways, and more. In this experiment, zebrafish (Danio rerio) received no treatment (control), or treatment with cannabinoids CBD or THC, and were then subjected to a novel tank diving test. Gills and brain tissue underwent RT-qPCR with target gene tp53 and housekeeping gene elfa. For statistical analyses, ∆∆Cq datawere used to normalize the expression of tp53 using qPCR, and expression levels were compared based on the treatment group. The data indicated no statistically significant difference in tp53 expression for zebrafish treated with CBD or THC.Due to time limit constraints, the expression for cannabinoid receptor genes cnr1 and cnr2 was not explored, however, primers for amplifying these genes were designed and optimized.
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