EQCN studies of protein adsorption at Pt and Au
LE3 .A278 2003
2003
Roscoe, Sharon
Acadia University
Master of Science
Masters
Chemistry
The interfacial behaviour of a range of proteins and organic acids was studied at 294 K in pH 7.4 phosphate buffer at polycrystalline Pt (mandelic acid, phenylalanine, mandelate racemase) as well as polycrystalline Au (mandelic acid, insulin, mandelate racemase, BSA) and in 0.05 M KClO4 at polycrystalline Au (mandelic acid, phenylalanine, insulin, BSA). Three electrochemical techniques, namely cyclic voltammetry (CV), electrochemical quartz crystal nanobalance (EQCN) and chronocoulometry (CC) were used to study the adsorption behaviour of these molecules. Using CV and CC, the surface charge density was determined and employed to obtain '_max' and '[Delta]GADS' values. In EQCN, the change in frequency was related to the change in mass at the electrode surface by the Sauerbrey equation, and in this way, '_max' and '[Delta]GADS' values were also obtained. Results from all techniques were fitted with the Langmuir adsorption isotherm. (Abstract shortened by UMI.)
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https://scholar.acadiau.ca/islandora/object/theses:2945
